imaging optimized 384 well plates Search Results


384-well clear-bottomed optical imaging plates  (Corning Life Sciences)
90
Corning Life Sciences 384-well clear-bottomed optical imaging plates
384 Well Clear Bottomed Optical Imaging Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imaging+optimized+384+well+plates/384+well+black+plastic+imaging+plates/pmc09094657-232-10-12
Average 90 stars, based on 1 article reviews
384-well clear-bottomed optical imaging plates - by Bioz Stars, 2026-10
90/100 stars
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386-well plate format corning 384-well high content imaging glass bottom microplate  (Corning Life Sciences)
90
Corning Life Sciences 386-well plate format corning 384-well high content imaging glass bottom microplate
386 Well Plate Format Corning 384 Well High Content Imaging Glass Bottom Microplate, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imaging+optimized+384+well+plates/386+well+plate+format+corning+384+well+high+content+imaging+glass+bottom+microplate/us12358960-1274-4-9
Average 90 stars, based on 1 article reviews
386-well plate format corning 384-well high content imaging glass bottom microplate - by Bioz Stars, 2026-10
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imaging-compatible 384-well plates  (Corning Life Sciences)
90
Corning Life Sciences imaging-compatible 384-well plates
The compound screening procedure. ( a ) Following assay development, stability and quality testing, the screening of the PCL against HAdV infection was performed. Imaging, image analysis and data processing were independently carried out at UZH and EPFL, before hit ranking. ( b ) Schematic overview of the wet-lab pipeline. PCL compounds and DFT positive control <t>in</t> <t>DMSO</t> as well as DMSO alone as negative control were pre-spotted onto <t>384-well</t> imaging plates by Echo acoustic liquid handling at 10 nl corresponding to a final concentration of 1.25 µM in 80 µl assay volume/well and stored at −20 °C. Compound-blinded plates are thawed and 4,000 A549 cells/wells seeded. The following day, the cells were inoculated with HAdV-C2-dE3B at 1.77*10 5 genome equivalents/well. Allowing for multiple viral replication rounds, the cells were PFA-fixed at 72 hpi and the nuclei stained with Hoechst 33342. The infection phenotypes were imaged using an epifluorescence HT microscope and scored using Plaque2.0. The data of the four technical replicates were further processed in R or through EPFL-BSF LIMS. ( c ) Exemplary epifluorescence microscopy images of cells in 384-wells stitched to a screening plate overview of 32 replicates of negative (two most left columns) and positive control (two most right columns) and 320 blinded PCL compounds (centre 20 columns). Hoechst-stained nuclei are shown in blue, viral GFP in green. ( d ) Representative 384-well epifluorescence microscopy images of the DMSO negative control (most left), the DFT positive control (most right) and the top hit Nelfinavir mesylate (centre). Empty black triangle indicates a plaque (infection focus) from a productively infected cell. White arrows point out infected cells that did not form a plaque. Hoechst-stained nuclei are shown in blue, infected cells expressing GFP in green. Scale bar is 5 mm.
Imaging Compatible 384 Well Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imaging+optimized+384+well+plates/imaging+compatible+384+well+plates/pmc07423605-121-27-29
Average 90 stars, based on 1 article reviews
imaging-compatible 384-well plates - by Bioz Stars, 2026-10
90/100 stars
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384 well streptavidin-coated sector® imager 2400 reader plates  (Meso Scale Diagnostics LLC)
90
Meso Scale Diagnostics LLC 384 well streptavidin-coated sector® imager 2400 reader plates
The compound screening procedure. ( a ) Following assay development, stability and quality testing, the screening of the PCL against HAdV infection was performed. Imaging, image analysis and data processing were independently carried out at UZH and EPFL, before hit ranking. ( b ) Schematic overview of the wet-lab pipeline. PCL compounds and DFT positive control <t>in</t> <t>DMSO</t> as well as DMSO alone as negative control were pre-spotted onto <t>384-well</t> imaging plates by Echo acoustic liquid handling at 10 nl corresponding to a final concentration of 1.25 µM in 80 µl assay volume/well and stored at −20 °C. Compound-blinded plates are thawed and 4,000 A549 cells/wells seeded. The following day, the cells were inoculated with HAdV-C2-dE3B at 1.77*10 5 genome equivalents/well. Allowing for multiple viral replication rounds, the cells were PFA-fixed at 72 hpi and the nuclei stained with Hoechst 33342. The infection phenotypes were imaged using an epifluorescence HT microscope and scored using Plaque2.0. The data of the four technical replicates were further processed in R or through EPFL-BSF LIMS. ( c ) Exemplary epifluorescence microscopy images of cells in 384-wells stitched to a screening plate overview of 32 replicates of negative (two most left columns) and positive control (two most right columns) and 320 blinded PCL compounds (centre 20 columns). Hoechst-stained nuclei are shown in blue, viral GFP in green. ( d ) Representative 384-well epifluorescence microscopy images of the DMSO negative control (most left), the DFT positive control (most right) and the top hit Nelfinavir mesylate (centre). Empty black triangle indicates a plaque (infection focus) from a productively infected cell. White arrows point out infected cells that did not form a plaque. Hoechst-stained nuclei are shown in blue, infected cells expressing GFP in green. Scale bar is 5 mm.
384 Well Streptavidin Coated Sector® Imager 2400 Reader Plates, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imaging+optimized+384+well+plates/384+well+sector++imager+standard+microtiter+plate/pmc07486241-294-2-0
Average 90 stars, based on 1 article reviews
384 well streptavidin-coated sector® imager 2400 reader plates - by Bioz Stars, 2026-10
90/100 stars
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384-well plates with flat bottom bd falcon imaging plates 353962  (Becton Dickinson)
90
Becton Dickinson 384-well plates with flat bottom bd falcon imaging plates 353962
The compound screening procedure. ( a ) Following assay development, stability and quality testing, the screening of the PCL against HAdV infection was performed. Imaging, image analysis and data processing were independently carried out at UZH and EPFL, before hit ranking. ( b ) Schematic overview of the wet-lab pipeline. PCL compounds and DFT positive control <t>in</t> <t>DMSO</t> as well as DMSO alone as negative control were pre-spotted onto <t>384-well</t> imaging plates by Echo acoustic liquid handling at 10 nl corresponding to a final concentration of 1.25 µM in 80 µl assay volume/well and stored at −20 °C. Compound-blinded plates are thawed and 4,000 A549 cells/wells seeded. The following day, the cells were inoculated with HAdV-C2-dE3B at 1.77*10 5 genome equivalents/well. Allowing for multiple viral replication rounds, the cells were PFA-fixed at 72 hpi and the nuclei stained with Hoechst 33342. The infection phenotypes were imaged using an epifluorescence HT microscope and scored using Plaque2.0. The data of the four technical replicates were further processed in R or through EPFL-BSF LIMS. ( c ) Exemplary epifluorescence microscopy images of cells in 384-wells stitched to a screening plate overview of 32 replicates of negative (two most left columns) and positive control (two most right columns) and 320 blinded PCL compounds (centre 20 columns). Hoechst-stained nuclei are shown in blue, viral GFP in green. ( d ) Representative 384-well epifluorescence microscopy images of the DMSO negative control (most left), the DFT positive control (most right) and the top hit Nelfinavir mesylate (centre). Empty black triangle indicates a plaque (infection focus) from a productively infected cell. White arrows point out infected cells that did not form a plaque. Hoechst-stained nuclei are shown in blue, infected cells expressing GFP in green. Scale bar is 5 mm.
384 Well Plates With Flat Bottom Bd Falcon Imaging Plates 353962, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imaging+optimized+384+well+plates/384+well+plates+with+flat+bottom+bd+falcon+imaging+plates+353962/pmc06350982-273-16-14
Average 90 stars, based on 1 article reviews
384-well plates with flat bottom bd falcon imaging plates 353962 - by Bioz Stars, 2026-10
90/100 stars
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cpc proliferation 384-well black imaging plates  (Corning Life Sciences)
90
Corning Life Sciences cpc proliferation 384-well black imaging plates
The compound screening procedure. ( a ) Following assay development, stability and quality testing, the screening of the PCL against HAdV infection was performed. Imaging, image analysis and data processing were independently carried out at UZH and EPFL, before hit ranking. ( b ) Schematic overview of the wet-lab pipeline. PCL compounds and DFT positive control <t>in</t> <t>DMSO</t> as well as DMSO alone as negative control were pre-spotted onto <t>384-well</t> imaging plates by Echo acoustic liquid handling at 10 nl corresponding to a final concentration of 1.25 µM in 80 µl assay volume/well and stored at −20 °C. Compound-blinded plates are thawed and 4,000 A549 cells/wells seeded. The following day, the cells were inoculated with HAdV-C2-dE3B at 1.77*10 5 genome equivalents/well. Allowing for multiple viral replication rounds, the cells were PFA-fixed at 72 hpi and the nuclei stained with Hoechst 33342. The infection phenotypes were imaged using an epifluorescence HT microscope and scored using Plaque2.0. The data of the four technical replicates were further processed in R or through EPFL-BSF LIMS. ( c ) Exemplary epifluorescence microscopy images of cells in 384-wells stitched to a screening plate overview of 32 replicates of negative (two most left columns) and positive control (two most right columns) and 320 blinded PCL compounds (centre 20 columns). Hoechst-stained nuclei are shown in blue, viral GFP in green. ( d ) Representative 384-well epifluorescence microscopy images of the DMSO negative control (most left), the DFT positive control (most right) and the top hit Nelfinavir mesylate (centre). Empty black triangle indicates a plaque (infection focus) from a productively infected cell. White arrows point out infected cells that did not form a plaque. Hoechst-stained nuclei are shown in blue, infected cells expressing GFP in green. Scale bar is 5 mm.
CPC Proliferation 384 Well Black Imaging Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imaging+optimized+384+well+plates/cpc+proliferation+384+well+black+imaging+plates/pmc04729552-127-0-6
Average 90 stars, based on 1 article reviews
cpc proliferation 384-well black imaging plates - by Bioz Stars, 2026-10
90/100 stars
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384 well optical imaging flat clear bottom black polystyrene tctreated micro plates  (Corning Life Sciences)
90
Corning Life Sciences 384 well optical imaging flat clear bottom black polystyrene tctreated micro plates
The compound screening procedure. ( a ) Following assay development, stability and quality testing, the screening of the PCL against HAdV infection was performed. Imaging, image analysis and data processing were independently carried out at UZH and EPFL, before hit ranking. ( b ) Schematic overview of the wet-lab pipeline. PCL compounds and DFT positive control <t>in</t> <t>DMSO</t> as well as DMSO alone as negative control were pre-spotted onto <t>384-well</t> imaging plates by Echo acoustic liquid handling at 10 nl corresponding to a final concentration of 1.25 µM in 80 µl assay volume/well and stored at −20 °C. Compound-blinded plates are thawed and 4,000 A549 cells/wells seeded. The following day, the cells were inoculated with HAdV-C2-dE3B at 1.77*10 5 genome equivalents/well. Allowing for multiple viral replication rounds, the cells were PFA-fixed at 72 hpi and the nuclei stained with Hoechst 33342. The infection phenotypes were imaged using an epifluorescence HT microscope and scored using Plaque2.0. The data of the four technical replicates were further processed in R or through EPFL-BSF LIMS. ( c ) Exemplary epifluorescence microscopy images of cells in 384-wells stitched to a screening plate overview of 32 replicates of negative (two most left columns) and positive control (two most right columns) and 320 blinded PCL compounds (centre 20 columns). Hoechst-stained nuclei are shown in blue, viral GFP in green. ( d ) Representative 384-well epifluorescence microscopy images of the DMSO negative control (most left), the DFT positive control (most right) and the top hit Nelfinavir mesylate (centre). Empty black triangle indicates a plaque (infection focus) from a productively infected cell. White arrows point out infected cells that did not form a plaque. Hoechst-stained nuclei are shown in blue, infected cells expressing GFP in green. Scale bar is 5 mm.
384 Well Optical Imaging Flat Clear Bottom Black Polystyrene Tctreated Micro Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imaging+optimized+384+well+plates/384+well+optical+imaging+flat+clear+bottom+black+polystyrene+tctreated+micro+plates/10__1002_slash_adbi__201800009-93-17-17
Average 90 stars, based on 1 article reviews
384 well optical imaging flat clear bottom black polystyrene tctreated micro plates - by Bioz Stars, 2026-10
90/100 stars
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384 well optical imaging plates #3985  (Corning Life Sciences)
90
Corning Life Sciences 384 well optical imaging plates #3985
The compound screening procedure. ( a ) Following assay development, stability and quality testing, the screening of the PCL against HAdV infection was performed. Imaging, image analysis and data processing were independently carried out at UZH and EPFL, before hit ranking. ( b ) Schematic overview of the wet-lab pipeline. PCL compounds and DFT positive control <t>in</t> <t>DMSO</t> as well as DMSO alone as negative control were pre-spotted onto <t>384-well</t> imaging plates by Echo acoustic liquid handling at 10 nl corresponding to a final concentration of 1.25 µM in 80 µl assay volume/well and stored at −20 °C. Compound-blinded plates are thawed and 4,000 A549 cells/wells seeded. The following day, the cells were inoculated with HAdV-C2-dE3B at 1.77*10 5 genome equivalents/well. Allowing for multiple viral replication rounds, the cells were PFA-fixed at 72 hpi and the nuclei stained with Hoechst 33342. The infection phenotypes were imaged using an epifluorescence HT microscope and scored using Plaque2.0. The data of the four technical replicates were further processed in R or through EPFL-BSF LIMS. ( c ) Exemplary epifluorescence microscopy images of cells in 384-wells stitched to a screening plate overview of 32 replicates of negative (two most left columns) and positive control (two most right columns) and 320 blinded PCL compounds (centre 20 columns). Hoechst-stained nuclei are shown in blue, viral GFP in green. ( d ) Representative 384-well epifluorescence microscopy images of the DMSO negative control (most left), the DFT positive control (most right) and the top hit Nelfinavir mesylate (centre). Empty black triangle indicates a plaque (infection focus) from a productively infected cell. White arrows point out infected cells that did not form a plaque. Hoechst-stained nuclei are shown in blue, infected cells expressing GFP in green. Scale bar is 5 mm.
384 Well Optical Imaging Plates #3985, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imaging+optimized+384+well+plates/384+well+optical+imaging+plates++3985/pmc06901385-119-0-0
Average 90 stars, based on 1 article reviews
384 well optical imaging plates #3985 - by Bioz Stars, 2026-10
90/100 stars
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elplasia 384-well microwell-patterned imaging plates  (Corning Life Sciences)
90
Corning Life Sciences elplasia 384-well microwell-patterned imaging plates
The compound screening procedure. ( a ) Following assay development, stability and quality testing, the screening of the PCL against HAdV infection was performed. Imaging, image analysis and data processing were independently carried out at UZH and EPFL, before hit ranking. ( b ) Schematic overview of the wet-lab pipeline. PCL compounds and DFT positive control <t>in</t> <t>DMSO</t> as well as DMSO alone as negative control were pre-spotted onto <t>384-well</t> imaging plates by Echo acoustic liquid handling at 10 nl corresponding to a final concentration of 1.25 µM in 80 µl assay volume/well and stored at −20 °C. Compound-blinded plates are thawed and 4,000 A549 cells/wells seeded. The following day, the cells were inoculated with HAdV-C2-dE3B at 1.77*10 5 genome equivalents/well. Allowing for multiple viral replication rounds, the cells were PFA-fixed at 72 hpi and the nuclei stained with Hoechst 33342. The infection phenotypes were imaged using an epifluorescence HT microscope and scored using Plaque2.0. The data of the four technical replicates were further processed in R or through EPFL-BSF LIMS. ( c ) Exemplary epifluorescence microscopy images of cells in 384-wells stitched to a screening plate overview of 32 replicates of negative (two most left columns) and positive control (two most right columns) and 320 blinded PCL compounds (centre 20 columns). Hoechst-stained nuclei are shown in blue, viral GFP in green. ( d ) Representative 384-well epifluorescence microscopy images of the DMSO negative control (most left), the DFT positive control (most right) and the top hit Nelfinavir mesylate (centre). Empty black triangle indicates a plaque (infection focus) from a productively infected cell. White arrows point out infected cells that did not form a plaque. Hoechst-stained nuclei are shown in blue, infected cells expressing GFP in green. Scale bar is 5 mm.
Elplasia 384 Well Microwell Patterned Imaging Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imaging+optimized+384+well+plates/elplasia+384+well+microwell+patterned+imaging+plates/pm40561077-60-53-55
Average 90 stars, based on 1 article reviews
elplasia 384-well microwell-patterned imaging plates - by Bioz Stars, 2026-10
90/100 stars
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image-compatible 384-well plates corning #4588  (Corning Life Sciences)
90
Corning Life Sciences image-compatible 384-well plates corning #4588
The compound screening procedure. ( a ) Following assay development, stability and quality testing, the screening of the PCL against HAdV infection was performed. Imaging, image analysis and data processing were independently carried out at UZH and EPFL, before hit ranking. ( b ) Schematic overview of the wet-lab pipeline. PCL compounds and DFT positive control <t>in</t> <t>DMSO</t> as well as DMSO alone as negative control were pre-spotted onto <t>384-well</t> imaging plates by Echo acoustic liquid handling at 10 nl corresponding to a final concentration of 1.25 µM in 80 µl assay volume/well and stored at −20 °C. Compound-blinded plates are thawed and 4,000 A549 cells/wells seeded. The following day, the cells were inoculated with HAdV-C2-dE3B at 1.77*10 5 genome equivalents/well. Allowing for multiple viral replication rounds, the cells were PFA-fixed at 72 hpi and the nuclei stained with Hoechst 33342. The infection phenotypes were imaged using an epifluorescence HT microscope and scored using Plaque2.0. The data of the four technical replicates were further processed in R or through EPFL-BSF LIMS. ( c ) Exemplary epifluorescence microscopy images of cells in 384-wells stitched to a screening plate overview of 32 replicates of negative (two most left columns) and positive control (two most right columns) and 320 blinded PCL compounds (centre 20 columns). Hoechst-stained nuclei are shown in blue, viral GFP in green. ( d ) Representative 384-well epifluorescence microscopy images of the DMSO negative control (most left), the DFT positive control (most right) and the top hit Nelfinavir mesylate (centre). Empty black triangle indicates a plaque (infection focus) from a productively infected cell. White arrows point out infected cells that did not form a plaque. Hoechst-stained nuclei are shown in blue, infected cells expressing GFP in green. Scale bar is 5 mm.
Image Compatible 384 Well Plates Corning #4588, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imaging+optimized+384+well+plates/image+compatible+384+well+plates+corning++4588/pm36464160-157-3-5
Average 90 stars, based on 1 article reviews
image-compatible 384-well plates corning #4588 - by Bioz Stars, 2026-10
90/100 stars
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384-well corning black/clear thin-bottom tissue-culture-treated imaging plates #3712  (Corning Life Sciences)
90
Corning Life Sciences 384-well corning black/clear thin-bottom tissue-culture-treated imaging plates #3712
The compound screening procedure. ( a ) Following assay development, stability and quality testing, the screening of the PCL against HAdV infection was performed. Imaging, image analysis and data processing were independently carried out at UZH and EPFL, before hit ranking. ( b ) Schematic overview of the wet-lab pipeline. PCL compounds and DFT positive control <t>in</t> <t>DMSO</t> as well as DMSO alone as negative control were pre-spotted onto <t>384-well</t> imaging plates by Echo acoustic liquid handling at 10 nl corresponding to a final concentration of 1.25 µM in 80 µl assay volume/well and stored at −20 °C. Compound-blinded plates are thawed and 4,000 A549 cells/wells seeded. The following day, the cells were inoculated with HAdV-C2-dE3B at 1.77*10 5 genome equivalents/well. Allowing for multiple viral replication rounds, the cells were PFA-fixed at 72 hpi and the nuclei stained with Hoechst 33342. The infection phenotypes were imaged using an epifluorescence HT microscope and scored using Plaque2.0. The data of the four technical replicates were further processed in R or through EPFL-BSF LIMS. ( c ) Exemplary epifluorescence microscopy images of cells in 384-wells stitched to a screening plate overview of 32 replicates of negative (two most left columns) and positive control (two most right columns) and 320 blinded PCL compounds (centre 20 columns). Hoechst-stained nuclei are shown in blue, viral GFP in green. ( d ) Representative 384-well epifluorescence microscopy images of the DMSO negative control (most left), the DFT positive control (most right) and the top hit Nelfinavir mesylate (centre). Empty black triangle indicates a plaque (infection focus) from a productively infected cell. White arrows point out infected cells that did not form a plaque. Hoechst-stained nuclei are shown in blue, infected cells expressing GFP in green. Scale bar is 5 mm.
384 Well Corning Black/Clear Thin Bottom Tissue Culture Treated Imaging Plates #3712, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imaging+optimized+384+well+plates/384+well+corning+black+clear+thin+bottom+tissue+culture+treated+imaging+plates++3712/10__2147_slash_ijhts__s10547-61-0-1
Average 90 stars, based on 1 article reviews
384-well corning black/clear thin-bottom tissue-culture-treated imaging plates #3712 - by Bioz Stars, 2026-10
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barcoded 384-well optical imaging plates  (Corning Life Sciences)
90
Corning Life Sciences barcoded 384-well optical imaging plates
The compound screening procedure. ( a ) Following assay development, stability and quality testing, the screening of the PCL against HAdV infection was performed. Imaging, image analysis and data processing were independently carried out at UZH and EPFL, before hit ranking. ( b ) Schematic overview of the wet-lab pipeline. PCL compounds and DFT positive control <t>in</t> <t>DMSO</t> as well as DMSO alone as negative control were pre-spotted onto <t>384-well</t> imaging plates by Echo acoustic liquid handling at 10 nl corresponding to a final concentration of 1.25 µM in 80 µl assay volume/well and stored at −20 °C. Compound-blinded plates are thawed and 4,000 A549 cells/wells seeded. The following day, the cells were inoculated with HAdV-C2-dE3B at 1.77*10 5 genome equivalents/well. Allowing for multiple viral replication rounds, the cells were PFA-fixed at 72 hpi and the nuclei stained with Hoechst 33342. The infection phenotypes were imaged using an epifluorescence HT microscope and scored using Plaque2.0. The data of the four technical replicates were further processed in R or through EPFL-BSF LIMS. ( c ) Exemplary epifluorescence microscopy images of cells in 384-wells stitched to a screening plate overview of 32 replicates of negative (two most left columns) and positive control (two most right columns) and 320 blinded PCL compounds (centre 20 columns). Hoechst-stained nuclei are shown in blue, viral GFP in green. ( d ) Representative 384-well epifluorescence microscopy images of the DMSO negative control (most left), the DFT positive control (most right) and the top hit Nelfinavir mesylate (centre). Empty black triangle indicates a plaque (infection focus) from a productively infected cell. White arrows point out infected cells that did not form a plaque. Hoechst-stained nuclei are shown in blue, infected cells expressing GFP in green. Scale bar is 5 mm.
Barcoded 384 Well Optical Imaging Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imaging+optimized+384+well+plates/barcoded+384+well+optical+imaging+plates/pmc10194851-136-41-47
Average 90 stars, based on 1 article reviews
barcoded 384-well optical imaging plates - by Bioz Stars, 2026-10
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Image Search Results


The compound screening procedure. ( a ) Following assay development, stability and quality testing, the screening of the PCL against HAdV infection was performed. Imaging, image analysis and data processing were independently carried out at UZH and EPFL, before hit ranking. ( b ) Schematic overview of the wet-lab pipeline. PCL compounds and DFT positive control in DMSO as well as DMSO alone as negative control were pre-spotted onto 384-well imaging plates by Echo acoustic liquid handling at 10 nl corresponding to a final concentration of 1.25 µM in 80 µl assay volume/well and stored at −20 °C. Compound-blinded plates are thawed and 4,000 A549 cells/wells seeded. The following day, the cells were inoculated with HAdV-C2-dE3B at 1.77*10 5 genome equivalents/well. Allowing for multiple viral replication rounds, the cells were PFA-fixed at 72 hpi and the nuclei stained with Hoechst 33342. The infection phenotypes were imaged using an epifluorescence HT microscope and scored using Plaque2.0. The data of the four technical replicates were further processed in R or through EPFL-BSF LIMS. ( c ) Exemplary epifluorescence microscopy images of cells in 384-wells stitched to a screening plate overview of 32 replicates of negative (two most left columns) and positive control (two most right columns) and 320 blinded PCL compounds (centre 20 columns). Hoechst-stained nuclei are shown in blue, viral GFP in green. ( d ) Representative 384-well epifluorescence microscopy images of the DMSO negative control (most left), the DFT positive control (most right) and the top hit Nelfinavir mesylate (centre). Empty black triangle indicates a plaque (infection focus) from a productively infected cell. White arrows point out infected cells that did not form a plaque. Hoechst-stained nuclei are shown in blue, infected cells expressing GFP in green. Scale bar is 5 mm.

Journal: Scientific Data

Article Title: A high-content image-based drug screen of clinical compounds against cell transmission of adenovirus

doi: 10.1038/s41597-020-00604-0

Figure Lengend Snippet: The compound screening procedure. ( a ) Following assay development, stability and quality testing, the screening of the PCL against HAdV infection was performed. Imaging, image analysis and data processing were independently carried out at UZH and EPFL, before hit ranking. ( b ) Schematic overview of the wet-lab pipeline. PCL compounds and DFT positive control in DMSO as well as DMSO alone as negative control were pre-spotted onto 384-well imaging plates by Echo acoustic liquid handling at 10 nl corresponding to a final concentration of 1.25 µM in 80 µl assay volume/well and stored at −20 °C. Compound-blinded plates are thawed and 4,000 A549 cells/wells seeded. The following day, the cells were inoculated with HAdV-C2-dE3B at 1.77*10 5 genome equivalents/well. Allowing for multiple viral replication rounds, the cells were PFA-fixed at 72 hpi and the nuclei stained with Hoechst 33342. The infection phenotypes were imaged using an epifluorescence HT microscope and scored using Plaque2.0. The data of the four technical replicates were further processed in R or through EPFL-BSF LIMS. ( c ) Exemplary epifluorescence microscopy images of cells in 384-wells stitched to a screening plate overview of 32 replicates of negative (two most left columns) and positive control (two most right columns) and 320 blinded PCL compounds (centre 20 columns). Hoechst-stained nuclei are shown in blue, viral GFP in green. ( d ) Representative 384-well epifluorescence microscopy images of the DMSO negative control (most left), the DFT positive control (most right) and the top hit Nelfinavir mesylate (centre). Empty black triangle indicates a plaque (infection focus) from a productively infected cell. White arrows point out infected cells that did not form a plaque. Hoechst-stained nuclei are shown in blue, infected cells expressing GFP in green. Scale bar is 5 mm.

Article Snippet: Ten nl of 10 mM PCL compounds, the nucleoside analogue DFT positive control (all dissolved in DMSO) and DMSO only as negative control were pre-spotted on imaging-compatible 384-well plates (Falcon plates, Corning Inc., New York, USA) using an Echo acoustic liquid handling system (Labcyte, San Jose, USA) by the EPFL-BSF, sealed and stored at −20 °C.

Techniques: Infection, Imaging, Positive Control, Negative Control, Concentration Assay, Staining, Microscopy, Epifluorescence Microscopy, Expressing